Ontario opened the scoring in the 25th minute, Reid Peardon tied it in the 40th minute, but Ontario tallied the winner four minutes later.
The Island’s girls’ squad lost 8-1 to Alberta with Emma Langley scoring for P.E.I.
P.E.I.’s boys play Saskatchewan today at 1 p.m. Atlantic while the girls play Nova Scotia at 3:30 p.m.
At the under-17 tourney in Fredericton, N.B., the Winsloe-Charlottetown Royals lost 6-1 to Manitoba.
Connor MacDonald scored for Prince Edward Island in the 17th minute to cut the Manitoba lead to 2-1, but Manitoba restored the two-goal lead a minute later.
Rinor Hoxha scored three in the win.
P.E.I. is off today and plays British Columbia Friday at 4 p.m.
The Island’s girls’ squad, the R.C. United, opens play today at 1 p.m. against the host New Brunswick.
In the senior men’s competition in Surrey, B.C., the Fredericton Reds lost 2-1 to Quebec. Four members of SoccerStop P.E.I. F.C. are playing for the Reds.
Fredericton is off today and plays Ontario Friday at 7 p.m.